Irwin appears in the episode “史前冰人” on the television program the boys are watching. He eventually comes to South Park to help capture 拉里 for 阿方斯·莫费斯托博士 and the federal agents. He fails in his attempt, resulting in his death, and Larry escapes in a helicopter to Des Moines.
Irwin also appeared in “人间地狱," as a resident of Hell. Satan holds a costume party, mistakes him for a patron at the party wearing a crocodile hunter costume, and deems it offensive. Steve tells Satan that he is the real crocodile hunter, causing Satan to throw him out of his party because he didn't have a costume on. Irwin's appearance in this episode was the source of controversy because it aired only weeks after his actual death by a stingray. Despite the fact that the rall stingray got away and Steve got killed from a helicopter blade on an earlier episode.
In the episode “冰球小英豪," Stan tries to convince the Park County Pee-Wee Hockey Team he is coaching that the Denver team won't kill them, and one of the players responds with, "That's what Steve Irwin said about those stingrays," referencing Irwin's cause of death.
His last appearance was in “第200集” where he, alongside other celebrities, launches a class-action lawsuit against the town of South Park. He still appears with a stingray sticking out of his heart.
He can usually be seen with a stingray in his chest, a direct reference to his accident. He wears tan shorts, a tan coat, and a dark brown hat lined with teeth. He has long blond hair and is rendered with an elongated head.